Setser, JW, Lingaraju, GM, Davis, CA, Samson, LD , and Drennan, CL (2012) Searching for DNA lesions: structural evidence for lower- and higher-affinity DNA binding conformations of human alkyladenine DNA glycosylase. Biochemistry. 2012 Jan 10;51(1):382-90.
Lingaraju ,GM, Davis, CA, Setser, JW, Drennan, CL, and Samson, LD (2011) Structural basis for the inhibition of human alkyladenine DNAglycosylase (AAG) by 3, N4-ethenocytosine containing DNA. J Biol Chem. Apr 15;286(15):13205-13.
Roma, GW, Crowley, LJ, Davis, CA, and Barber, MJ. (2005) Mutagenesis of Glycine 179 Modulates Both Catalytic Efficiency and Reduced Pyridine Nucleotide Specificity in Cytochrome b(5) Reductase. Biochemistry. 44(41):13467-76.
Percy, MJ, Crowley, LJ, Davis, CA, McMullin, MF, Savage G, Hughes J, McMahon, C, Quinn, RJ, Smith, O, Barber, MJ, Lappin, TR. (2005) Recessive congenital methaemoglobinaemia: functional characterization of the novel D239G mutation in the NADH-binding lobe of cytochrome b reductase. Br J Haematology 129(6):847-53.
Marohnic, CC, Crowley, LJ, Davis, CA, Smith, ET, and Barber, MJ. (2005) Cytochrome b5 Reductase: The Role of the si-Face Residues, Proline 92 and Tyrosine 93, in Structure and Catalysis. Biochemistry 44(7):2449-61.
Davis, CA, Crowley, LJ, and Barber, MJ (2004) Cytochrome b5 reductase: the roles of the recessive congenital methemoglobinemia mutants P144L, L148P, and R159*. Arch Biochem Biophys. 431(2):233-44.
Davis, CA and Barber, MJ. (2004) Cytochrome b5 oxidoreductase: expression and characterization of the original familial ideopathic methemoglobinemia mutations E255- and G291D. Arch Biochem Biophys. 425(2):123-32.
Smith, ET, Davis, CA, and Barber, MJ. (2003) Voltammetric simulations of multiple electron transfer/proton transfer coupled reactions: flavin adenine dinucleotide as a model system. Anal Biochem. 323(1):114-21.
Bewley, MC, Davis, CA, Marohnic, CC, Taormina, D, and Barber, MJ. (2003) The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site. Biochemistry. 42(45):13145-51.
Davis, CA and Barber, MJ. (2003) Heterologous expression of enzymopenic methemoglobinemia variants using a novel NADH:cytochrome c reductase fusion protein. Protein Expr Purif. 30(1):43-54.
Davis, CA, Dhawan, IK, Johnson, MK, Barber, MJ. (2002) Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein: identification of histidines 62 and 85 as the heme axial ligands. Arch Biochem Biophys. 400(1):63-75
Associate Professor of Biology and Chemistry
Current research interests:
My research endeavors since joining the B-CU faculty in 2008 involve techniques and methods in the areas of molecular biochemistry, structural enzymology, and synthetic biology. My projects include a wide-array of protein purification, expression, and characterization techniques, hoping to engage undergraduates aspiring to become professionals in the biological sciences and immerse them in an intense and fulfilling introduction to the scientific method. My projects revolve around proteins that are involved in disulfide-exchange, electron transport, DNA repair, biosynthesis of deoxyribonucleotides, and engineering of microbes. These areas are fundamental to the overall daily cellular maintenance, the prevention of cellular damage, and to understanding evolving techniques in biology. My ongoing projects will also involve collaborations with Dr. Sean Elliott at Boston University, HHMI Professor and investigator Catherine Drennan at the Massachusetts Institute of Technology, Dr. Julie Maupin-Furlow at the University of Florida, and faculty at B-CU including Dr. Raphael Isokpehi, Dr. Danyell Wilson and Dr. Vishwa Trivedi.
Dr. Davis has also been involved with utilizing synthetic biology techniques to study heavy metal contamination. Right now the focus is on ways to mitigate copper ion concentrations in local freshwater bodies of water. This has been an emerging field for the last decade and has become is extremely useful for creating innovative ways to analyze and promote problem solving through basic science. This ongoing project has been supported by the B-CU Center for Undergraduate Research Excellence (CURE) as well as through NSF.
Recently, Dr. Davis has started to use systematic reviews as a way to train undergraduates to analyze the massive quantity of data available in the world today. Systematic reviews provide another avenue to explore scientific inquiry, communication, and interpretation of data. This method becomes an effective tool to help students understand that the amount of data generated needs to be critically evaluated on multiple levels in order to find new research questions and avenues for discussion. Currently students are studying the role of thioredoxin binding/interacting in DNA metabolic pathways. These efforts are in collaboration with Dr. Danyell Wilson at where undergraduates are directly involved and is supported by the Florida-Georgia Louis Stokes Alliance for Minority Participation (FGLSAMP-NSF) and CURE (B-CU).